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Addgene inc shctnnb1
Shctnnb1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shctnnb1/shCTNNB1+%23+1+(Plasmid+%2342543)/pm36150916-76-8-7
Average 91 stars, based on 3 article reviews
shctnnb1 - by Bioz Stars, 2026-09
91/100 stars

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Mutagenesis:

Article Title: Axitinib blocks Wnt/β-catenin signaling and directs asymmetric cell division in cancer
Article Snippet: .. Super 8xTOPFlash (12456), Super 8xFOPFlash (TOPFlash mutant) (12457), pRL-SV40P (27163), 7xTcf-eGFP//SV40-mCherry (7TGC) (24304), pcDNA3-β-catenin (16828), β-catenin-ΔN47 (19287), β-catenin-4A (S33A/S37A/T41A/S45A) (24204), sh-CTNNB1-1248 (19761), sh-CTNNB1-2279 (19762), shCTNNB1 #1 (42543), pMD2.G (12259), and pCMVR8.74 (22036) were obtained from Addgene. ..

shRNA:

Article Title: The role of CTNNB1 mutations and matrix metalloproteinases (MMPs) in anti-angiogenesis treatment of endometrial carcinoma.
Article Snippet: Cells were transiently transfected using Promega FuGENE® HD Transfection Reagent (Promega, Madison, WI) according to manufacturer recommended protocols. .. Select lentiviral vectors were purchased directly from Addgene: shCTNNB1 #1 (42543), shCTNNB1 #2 (42544), Scramble shRNA (1864), pcw107-V5 (62511), and beta-catenin-S33Y-pcw107-V5 (64616). pLX303-CTTNB1-GFP, pLX303-CTNNB1-Δ90, and pLX303-GFP vectors were generated by cloning the insert gene frompEGFP-N1-Flag (Addgene 60360), CTNNB1-GFP (Addgene 71367), or pENTR-N90-beta-catenin del90-bCatenin (Addgene 31787) into a pLX303 vector plasmid (Addgene 25897) using Gateway TOPO cloning (Thermo Fisher Scientific). .. For stable transduction, 293 T cells were co-transfected with target gene vector (3 μg), psPAX2 packaging plasmid (5 μg, Addgene 12260), and pMD2.G enveloped plasmid (2.5 μg, Addgene 12259) using FuGENE® HD Transfection Reagent (Promega).

Article Title: A tumor suppressor enhancing module orchestrated by GATA4 denotes a therapeutic opportunity for GATA4 deficient HCC patients
Article Snippet: .. Following shRNAs were purchased from the Sigma Mission shRNA Library: shGATA4 (TRCN0000020424), shCTNNB1 (TRCN0000314920), and Luciferase shRNA (shLuc, SHC007). psPAX2, pMD2.G, pCAG-IRES-Neo and pLKO.1-puro were purchased from Addgene. .. The pLVX-TetOne-Puro plasmid was purchased from Clontech.

Article Title: The Assessment of Glioblastoma Tumorspheres Reveals Molecular Determinants of Proliferation and Therapeutic Response
Article Snippet: The secondary antibody used was HRP conjugated anti rabbit (Cell Signaling). .. 199 shRNA Knockdown: Plasmids with shRICTOR (Addgene plasmid 1853, David Sabatini, (Sarbassov et al., 2005)), shRAPTOR (Addgene plasmid 1857, David Sabatini,(Sarbassov et al., 2005)), shGSK3B#1 (Addgene plasmid 32496 , Alex Toker,(Yoeli-Lerner et al., 2009)), and shCTNNB1 (Addgene plasmid 18803, Bob Weinberg,(Onder et al., 2008)) constructs, cloned into pLKO expression vectors, were purchased from Addgene. ..

Generated:

Article Title: The role of CTNNB1 mutations and matrix metalloproteinases (MMPs) in anti-angiogenesis treatment of endometrial carcinoma.
Article Snippet: Cells were transiently transfected using Promega FuGENE® HD Transfection Reagent (Promega, Madison, WI) according to manufacturer recommended protocols. .. Select lentiviral vectors were purchased directly from Addgene: shCTNNB1 #1 (42543), shCTNNB1 #2 (42544), Scramble shRNA (1864), pcw107-V5 (62511), and beta-catenin-S33Y-pcw107-V5 (64616). pLX303-CTTNB1-GFP, pLX303-CTNNB1-Δ90, and pLX303-GFP vectors were generated by cloning the insert gene frompEGFP-N1-Flag (Addgene 60360), CTNNB1-GFP (Addgene 71367), or pENTR-N90-beta-catenin del90-bCatenin (Addgene 31787) into a pLX303 vector plasmid (Addgene 25897) using Gateway TOPO cloning (Thermo Fisher Scientific). .. For stable transduction, 293 T cells were co-transfected with target gene vector (3 μg), psPAX2 packaging plasmid (5 μg, Addgene 12260), and pMD2.G enveloped plasmid (2.5 μg, Addgene 12259) using FuGENE® HD Transfection Reagent (Promega).

Cloning:

Article Title: The role of CTNNB1 mutations and matrix metalloproteinases (MMPs) in anti-angiogenesis treatment of endometrial carcinoma.
Article Snippet: Cells were transiently transfected using Promega FuGENE® HD Transfection Reagent (Promega, Madison, WI) according to manufacturer recommended protocols. .. Select lentiviral vectors were purchased directly from Addgene: shCTNNB1 #1 (42543), shCTNNB1 #2 (42544), Scramble shRNA (1864), pcw107-V5 (62511), and beta-catenin-S33Y-pcw107-V5 (64616). pLX303-CTTNB1-GFP, pLX303-CTNNB1-Δ90, and pLX303-GFP vectors were generated by cloning the insert gene frompEGFP-N1-Flag (Addgene 60360), CTNNB1-GFP (Addgene 71367), or pENTR-N90-beta-catenin del90-bCatenin (Addgene 31787) into a pLX303 vector plasmid (Addgene 25897) using Gateway TOPO cloning (Thermo Fisher Scientific). .. For stable transduction, 293 T cells were co-transfected with target gene vector (3 μg), psPAX2 packaging plasmid (5 μg, Addgene 12260), and pMD2.G enveloped plasmid (2.5 μg, Addgene 12259) using FuGENE® HD Transfection Reagent (Promega).

Plasmid Preparation:

Article Title: The role of CTNNB1 mutations and matrix metalloproteinases (MMPs) in anti-angiogenesis treatment of endometrial carcinoma.
Article Snippet: Cells were transiently transfected using Promega FuGENE® HD Transfection Reagent (Promega, Madison, WI) according to manufacturer recommended protocols. .. Select lentiviral vectors were purchased directly from Addgene: shCTNNB1 #1 (42543), shCTNNB1 #2 (42544), Scramble shRNA (1864), pcw107-V5 (62511), and beta-catenin-S33Y-pcw107-V5 (64616). pLX303-CTTNB1-GFP, pLX303-CTNNB1-Δ90, and pLX303-GFP vectors were generated by cloning the insert gene frompEGFP-N1-Flag (Addgene 60360), CTNNB1-GFP (Addgene 71367), or pENTR-N90-beta-catenin del90-bCatenin (Addgene 31787) into a pLX303 vector plasmid (Addgene 25897) using Gateway TOPO cloning (Thermo Fisher Scientific). .. For stable transduction, 293 T cells were co-transfected with target gene vector (3 μg), psPAX2 packaging plasmid (5 μg, Addgene 12260), and pMD2.G enveloped plasmid (2.5 μg, Addgene 12259) using FuGENE® HD Transfection Reagent (Promega).

Article Title: The Assessment of Glioblastoma Tumorspheres Reveals Molecular Determinants of Proliferation and Therapeutic Response
Article Snippet: The secondary antibody used was HRP conjugated anti rabbit (Cell Signaling). .. 199 shRNA Knockdown: Plasmids with shRICTOR (Addgene plasmid 1853, David Sabatini, (Sarbassov et al., 2005)), shRAPTOR (Addgene plasmid 1857, David Sabatini,(Sarbassov et al., 2005)), shGSK3B#1 (Addgene plasmid 32496 , Alex Toker,(Yoeli-Lerner et al., 2009)), and shCTNNB1 (Addgene plasmid 18803, Bob Weinberg,(Onder et al., 2008)) constructs, cloned into pLKO expression vectors, were purchased from Addgene. ..

Luciferase:

Article Title: A tumor suppressor enhancing module orchestrated by GATA4 denotes a therapeutic opportunity for GATA4 deficient HCC patients
Article Snippet: .. Following shRNAs were purchased from the Sigma Mission shRNA Library: shGATA4 (TRCN0000020424), shCTNNB1 (TRCN0000314920), and Luciferase shRNA (shLuc, SHC007). psPAX2, pMD2.G, pCAG-IRES-Neo and pLKO.1-puro were purchased from Addgene. .. The pLVX-TetOne-Puro plasmid was purchased from Clontech.

Knockdown:

Article Title: The Assessment of Glioblastoma Tumorspheres Reveals Molecular Determinants of Proliferation and Therapeutic Response
Article Snippet: The secondary antibody used was HRP conjugated anti rabbit (Cell Signaling). .. 199 shRNA Knockdown: Plasmids with shRICTOR (Addgene plasmid 1853, David Sabatini, (Sarbassov et al., 2005)), shRAPTOR (Addgene plasmid 1857, David Sabatini,(Sarbassov et al., 2005)), shGSK3B#1 (Addgene plasmid 32496 , Alex Toker,(Yoeli-Lerner et al., 2009)), and shCTNNB1 (Addgene plasmid 18803, Bob Weinberg,(Onder et al., 2008)) constructs, cloned into pLKO expression vectors, were purchased from Addgene. ..

Construct:

Article Title: The Assessment of Glioblastoma Tumorspheres Reveals Molecular Determinants of Proliferation and Therapeutic Response
Article Snippet: The secondary antibody used was HRP conjugated anti rabbit (Cell Signaling). .. 199 shRNA Knockdown: Plasmids with shRICTOR (Addgene plasmid 1853, David Sabatini, (Sarbassov et al., 2005)), shRAPTOR (Addgene plasmid 1857, David Sabatini,(Sarbassov et al., 2005)), shGSK3B#1 (Addgene plasmid 32496 , Alex Toker,(Yoeli-Lerner et al., 2009)), and shCTNNB1 (Addgene plasmid 18803, Bob Weinberg,(Onder et al., 2008)) constructs, cloned into pLKO expression vectors, were purchased from Addgene. ..

Clone Assay:

Article Title: The Assessment of Glioblastoma Tumorspheres Reveals Molecular Determinants of Proliferation and Therapeutic Response
Article Snippet: The secondary antibody used was HRP conjugated anti rabbit (Cell Signaling). .. 199 shRNA Knockdown: Plasmids with shRICTOR (Addgene plasmid 1853, David Sabatini, (Sarbassov et al., 2005)), shRAPTOR (Addgene plasmid 1857, David Sabatini,(Sarbassov et al., 2005)), shGSK3B#1 (Addgene plasmid 32496 , Alex Toker,(Yoeli-Lerner et al., 2009)), and shCTNNB1 (Addgene plasmid 18803, Bob Weinberg,(Onder et al., 2008)) constructs, cloned into pLKO expression vectors, were purchased from Addgene. ..

Expressing:

Article Title: The Assessment of Glioblastoma Tumorspheres Reveals Molecular Determinants of Proliferation and Therapeutic Response
Article Snippet: The secondary antibody used was HRP conjugated anti rabbit (Cell Signaling). .. 199 shRNA Knockdown: Plasmids with shRICTOR (Addgene plasmid 1853, David Sabatini, (Sarbassov et al., 2005)), shRAPTOR (Addgene plasmid 1857, David Sabatini,(Sarbassov et al., 2005)), shGSK3B#1 (Addgene plasmid 32496 , Alex Toker,(Yoeli-Lerner et al., 2009)), and shCTNNB1 (Addgene plasmid 18803, Bob Weinberg,(Onder et al., 2008)) constructs, cloned into pLKO expression vectors, were purchased from Addgene. ..



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